Hotspot

ycf1

SSC · 123980-129835 · 5856 bp

Back to Trillium

Pi 0.0795

MarkerSeek score 76.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5856
variable_sites: 695
pi: 0.0795
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTTGCGTCTTTGAGCACG	ACGGAAAAACTATGCCACGA	45.0	45.0	58.0	57.8	1106-1118 bp	1.0	90.3
ycf1_p2	ATTTTGCGTCTTTGAGCACG	TGCCTGCCCAAAATGTATGA	45.0	45.0	58.0	57.8	929-941 bp	1.0	89.9
ycf1_p3	AAAAGTGGGGAATGCACACT	ACGGAAAAACTATGCCACGA	45.0	45.0	57.9	57.8	1151 bp	0.5	57.9
ycf1_p4	TCTCCTGTTTCGCCTTTTCC	ACGGAAAAACTATGCCACGA	50.0	45.0	58.1	57.8	787 bp	0.5	57.8
ycf1_p5	CTCAGCCTGCATTTTCTCCT	ACGGAAAAACTATGCCACGA	50.0	45.0	57.9	57.8	810 bp	0.5	57.6