Hotspot

atpE

LSC · 52619-53023 · 405 bp

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Pi 0.295

MarkerSeek score 58.80

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 179
pi: 0.295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	CAAATCGATGGCGGGTTCTA	TTGGTTTGACTGCCCTAACC	50.0	50.0	58.1	58.0	1801 bp	0.5	59.5
atpE_p2	TCGATGGCGGGTTCTATTTG	TTGGTTTGACTGCCCTAACC	50.0	50.0	58.1	58.0	1797 bp	0.5	59.5
atpE_p3	CAAATCGATGGCGGGTTCTA	GCAGTTTATGTACCTGCGGA	50.0	50.0	58.1	58.0	1571 bp	0.5	59.4
atpE_p4	TCGATGGCGGGTTCTATTTG	GCAGTTTATGTACCTGCGGA	50.0	50.0	58.1	58.0	1567 bp	0.5	59.4
atpE_p5	TGGCATCTGAATTTGCACCT	TTGGTTTGACTGCCCTAACC	45.0	50.0	58.1	58.0	1779 bp	0.5	59.3