Hotspot

ycf1-trnN-GUU

IRa · 129836-130411 · 576 bp

Back to Trillium

Pi 0.109

MarkerSeek score 84.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 576
variable_sites: 94
pi: 0.109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	CCTTCTTCCATAACCCGAGC	GAGCGAGCCCTTTAATTCCA	55.0	50.0	58.0	58.0	822-994 bp	1.0	90.1
ycf1-trnN-GUU_p2	CCTTCTTCCATAACCCGAGC	TAGCATAGCAAAGACGCGTT	55.0	45.0	58.0	58.0	1086-1258 bp	1.0	88.4
ycf1-trnN-GUU_p3	CCTTCTTCCATAACCCGAGC	GTAACCCAGGCCAGAATCAG	55.0	55.0	58.0	58.0	1182-1359 bp	1.0	87.4
ycf1-trnN-GUU_p4	CCTTCTTCCATAACCCGAGC	TCGAACTTGGGTAGGGTCTT	55.0	50.0	58.0	58.0	1268-1445 bp	1.0	87.2
ycf1-trnN-GUU_p5	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	55.0	50.0	58.0	58.0	1117-1134 bp	0.5	60.6