Hotspot

ndhF-rpl32

SSC · 112520-113290 · 771 bp

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Pi 0.022

MarkerSeek score 61.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 771
variable_sites: 25
pi: 0.022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTGGGAATAATCAGA	AGAAAGAGCTTTTACTGCAGT	40.9	38.1	57.7	55.4	938-970 bp	1.0	45.3
ndhF-rpl32_p2	TGTTGCTGTGGGAATAATCAGA	AAGAAAGAGCTTTTACTGCAGT	40.9	36.4	57.7	56.2	939-971 bp	1.0	45.3
ndhF-rpl32_p3	TGTTGCTGTGGGAATAATCAGA	AGAAAGAGCTTTTACTGCAGTT	40.9	36.4	57.7	56.2	938-970 bp	1.0	45.3
ndhF-rpl32_p4	TGCTGTGGGAATAATCAGAAGT	AGAAAGAGCTTTTACTGCAGT	40.9	38.1	57.4	55.4	935-967 bp	1.0	45.3
ndhF-rpl32_p5	TGTTGCTGTGGGAATAATCAGA	GCTAAAGAAAGAGCTTTTACTGCA	40.9	37.5	57.7	58.0	943-975 bp	1.0	45.3