Hotspot

trnN-GUU-ycf1

IRb · 109499-110074 · 576 bp

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Pi 0.0346

MarkerSeek score 67.50

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 576
variable_sites: 29
pi: 0.0346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AGTCAGTTAACAGCCAACCG	AGGAAGAGATAAGAGGGCCC	50.0	55.0	58.1	57.9	709-726 bp	0.5	60.0
trnN-GUU-ycf1_p2	AGTCAGTTAACAGCCAACCG	GGAAGAGATAAGAGGGCCCT	50.0	55.0	58.1	57.9	708-725 bp	0.5	60.0
trnN-GUU-ycf1_p3	ACGACCAGTCAGTTAACAGC	AGGAAGAGATAAGAGGGCCC	50.0	55.0	57.8	57.9	715-732 bp	0.5	59.8
trnN-GUU-ycf1_p4	ACGACCAGTCAGTTAACAGC	GGAAGAGATAAGAGGGCCCT	50.0	55.0	57.8	57.9	714-731 bp	0.5	59.8
trnN-GUU-ycf1_p5	AGGATTCGAACCTACGACCA	GGAAGAGATAAGAGGGCCCT	50.0	55.0	58.2	57.9	727-744 bp	0.5	59.7