Hotspot

rbcL-accD

LSC · 56645-57467 · 823 bp

Back to Trillium

Pi 0.176

MarkerSeek score 74.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 218
pi: 0.176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAAGGGGAACGTGAGATGAC	CTCAGCCCACACCTATGTTC	55.0	55.0	58.0	58.0	1317 bp	0.5	59.7
rbcL-accD_p2	GGCTTCAGGGGGTATTCATG	CTCAGCCCACACCTATGTTC	55.0	55.0	58.0	58.0	1192 bp	0.5	59.7
rbcL-accD_p3	GAAGGGGAACGTGAGATGAC	GCCCACACCTATGTTCTAGC	55.0	55.0	58.0	58.0	1313 bp	0.5	59.5
rbcL-accD_p4	CGTGAAGCTTGCAAATGGAG	CTCAGCCCACACCTATGTTC	50.0	55.0	58.0	58.0	987 bp	0.5	59.5
rbcL-accD_p5	GGCTTCAGGGGGTATTCATG	GCCCACACCTATGTTCTAGC	55.0	55.0	58.0	58.0	1188 bp	0.5	59.5