Hotspot

ndhC-trnV-UAC

LSC · 50633-51515 · 883 bp

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Pi 0.0534

MarkerSeek score 71.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 71
pi: 0.0534
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTCACTAAAGAAGGGGCCA	CAAATAGAACCCGCCATCGA	50.0	50.0	58.0	58.1	2054-2270 bp	0.5	59.3
ndhC-trnV-UAC_p2	TCTCACTAAAGAAGGGGCCA	TAGAACCCGCCATCGATTTG	50.0	50.0	58.0	58.1	2050-2266 bp	0.5	59.3
ndhC-trnV-UAC_p3	TCTCACTAAAGAAGGGGCCA	AGGTGCAAATTCAGATGCCA	50.0	45.0	58.0	58.1	2072-2288 bp	0.5	59.1
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	CAAATAGAACCCGCCATCGA	45.0	50.0	58.1	58.1	2078-2294 bp	0.5	59.0
ndhC-trnV-UAC_p5	TGGTTCAGGCATTTGCTCAT	TAGAACCCGCCATCGATTTG	45.0	50.0	58.1	58.1	2074-2290 bp	0.5	59.0