Hotspot

trnF-GAA-ndhJ

LSC · 48249-48942 · 694 bp

Back to Trillium

Pi 0.00649

MarkerSeek score 64.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 7
pi: 0.00649
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCTGAAAGTTGGGTAGGCTG	50.0	55.0	58.1	58.2	776-792 bp	1.0	81.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CTGAAAGTTGGGTAGGCTGG	50.0	55.0	58.1	58.2	775-791 bp	1.0	81.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TTGATGCCTGAAAGTTGGGT	50.0	45.0	58.1	57.6	782-798 bp	1.0	79.6
trnF-GAA-ndhJ_p4	GGGATAGCTCAGTCGGTAGA	CTGAAAGTTGGGTAGGCTGG	55.0	55.0	57.7	58.2	811-827 bp	1.0	79.4
trnF-GAA-ndhJ_p5	GGGATAGCTCAGTCGGTAGA	CCTGAAAGTTGGGTAGGCTG	55.0	55.0	57.7	58.2	812-828 bp	1.0	79.3