primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CCTGAAAGTTGGGTAGGCTG	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.225511	48249	48942	776-792	1.000000	81.3
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CTGAAAGTTGGGTAGGCTGG	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.225511	48249	48942	775-791	1.000000	81.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TTGATGCCTGAAAGTTGGGT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.431960	48249	48942	782-798	1.000000	79.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GGGATAGCTCAGTCGGTAGA	CTGAAAGTTGGGTAGGCTGG	20	20	55.0	55.0	57.7	58.2	0.0	0.0	0.447153	48249	48942	811-827	1.000000	79.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGGATAGCTCAGTCGGTAGA	CCTGAAAGTTGGGTAGGCTG	20	20	55.0	55.0	57.7	58.2	0.0	0.0	0.447153	48249	48942	812-828	1.000000	79.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCTCACTAAAGAAGGGGCCA	CAAATAGAACCCGCCATCGA	20	20	50.0	50.0	58.0	58.1	4.4	0.0	0.090759	50633	51515	2054-2270	0.500000	59.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCTCACTAAAGAAGGGGCCA	TAGAACCCGCCATCGATTTG	20	20	50.0	50.0	58.0	58.1	4.4	0.0	0.090759	50633	51515	2050-2266	0.500000	59.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCTCACTAAAGAAGGGGCCA	AGGTGCAAATTCAGATGCCA	20	20	50.0	45.0	58.0	58.1	4.4	0.0	0.111376	50633	51515	2072-2288	0.500000	59.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	CAAATAGAACCCGCCATCGA	20	20	45.0	50.0	58.1	58.1	2.1	0.0	0.124408	50633	51515	2078-2294	0.500000	59.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGTTCAGGCATTTGCTCAT	TAGAACCCGCCATCGATTTG	20	20	45.0	50.0	58.1	58.1	2.1	0.0	0.124408	50633	51515	2074-2290	0.500000	59.0
trnV-UAC_p1	trnV-UAC	1	ACAAAACTTTCTCGCTCGGT	CTAGGACACGAGTAGAGGCA	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.090353	51516	52186	1770-1986	0.500000	59.3
trnV-UAC_p2	trnV-UAC	2	ACAAAACTTTCTCGCTCGGT	CCCTATTGCCACAGCTGTAG	20	20	45.0	55.0	58.1	58.0	0.0	21.2	0.091493	51516	52186	2021-2237	0.500000	59.3
trnV-UAC_p3	trnV-UAC	3	TTCTATGGACCAAGCAACCG	CTAGGACACGAGTAGAGGCA	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.147768	51516	52186	1247-1247	0.500000	58.8
trnV-UAC_p4	trnV-UAC	4	TTCTATGGACCAAGCAACCG	CCCTATTGCCACAGCTGTAG	20	20	50.0	55.0	57.9	58.0	0.0	21.2	0.148908	51516	52186	1498-1498	0.500000	58.8
trnV-UAC_p5	trnV-UAC	5	ACAAAACTTTCTCGCTCGGT	ACATTGATCCGCAAGAAGCT	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.170336	51516	52186	1878-2094	0.500000	58.7
atpE_p1	atpE	1	CAAATCGATGGCGGGTTCTA	TTGGTTTGACTGCCCTAACC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.062189	52619	53023	1801-1801	0.500000	59.5
atpE_p2	atpE	2	TCGATGGCGGGTTCTATTTG	TTGGTTTGACTGCCCTAACC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.062189	52619	53023	1797-1797	0.500000	59.5
atpE_p3	atpE	3	CAAATCGATGGCGGGTTCTA	GCAGTTTATGTACCTGCGGA	20	20	50.0	50.0	58.1	58.0	0.0	5.5	0.070438	52619	53023	1571-1571	0.500000	59.4
atpE_p4	atpE	4	TCGATGGCGGGTTCTATTTG	GCAGTTTATGTACCTGCGGA	20	20	50.0	50.0	58.1	58.0	0.0	5.5	0.070438	52619	53023	1567-1567	0.500000	59.4
atpE_p5	atpE	5	TGGCATCTGAATTTGCACCT	TTGGTTTGACTGCCCTAACC	20	20	45.0	50.0	58.1	58.0	4.3	0.0	0.082806	52619	53023	1779-1779	0.500000	59.3
atpB_p1	atpB	1	TGCCTCTACTCGTGTCCTAG	TATATGGCGCAACCCAATCC	20	20	55.0	50.0	58.0	58.1	0.0	10.8	0.135531	53020	54516	2380-2380	0.500000	58.9
atpB_p2	atpB	2	CTACAGCTGTGGCAATAGGG	TATATGGCGCAACCCAATCC	20	20	55.0	50.0	58.0	58.1	29.6	10.8	0.136672	53020	54516	2129-2129	0.500000	58.9
atpB_p3	atpB	3	TGCCTCTACTCGTGTCCTAG	TGGCCGTTGAAACCGAAATA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.146274	53020	54516	2161-2161	0.500000	58.8
atpB_p4	atpB	4	CTACAGCTGTGGCAATAGGG	TGGCCGTTGAAACCGAAATA	20	20	55.0	45.0	58.0	58.1	29.6	0.0	0.147414	53020	54516	1910-1910	0.500000	58.8
atpB_p5	atpB	5	TGCCTCTACTCGTGTCCTAG	GGTGACATAAGTCCCTCCCT	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.158877	53020	54516	2579-2579	0.500000	58.8
atpB-rbcL_p1	atpB-rbcL	1	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006913	54517	55210	1653-1653	0.500000	60.0
atpB-rbcL_p2	atpB-rbcL	2	GCCGACTGGAACACTTAGAG	AGATCCTCCAGACGTAGAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	54517	55210	1416-1416	0.500000	59.9
atpB-rbcL_p3	atpB-rbcL	3	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	20	20	55.0	55.0	58.0	58.0	0.0	5.3	0.026671	54517	55210	1414-1414	0.500000	59.9
atpB-rbcL_p4	atpB-rbcL	4	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	54517	55210	1415-1415	0.500000	59.9
atpB-rbcL_p5	atpB-rbcL	5	GCCGACTGGAACACTTAGAG	TCTCGATCTGGTAGCATCGT	20	20	55.0	50.0	58.0	58.0	0.0	15.3	0.033627	54517	55210	1268-1268	0.500000	59.9
rbcL-accD_p1	rbcL-accD	1	GAAGGGGAACGTGAGATGAC	CTCAGCCCACACCTATGTTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.041378	56645	57467	1317-1317	0.500000	59.7
rbcL-accD_p2	rbcL-accD	2	GGCTTCAGGGGGTATTCATG	CTCAGCCCACACCTATGTTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.042956	56645	57467	1192-1192	0.500000	59.7
rbcL-accD_p3	rbcL-accD	3	GAAGGGGAACGTGAGATGAC	GCCCACACCTATGTTCTAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.056613	56645	57467	1313-1313	0.500000	59.5
rbcL-accD_p4	rbcL-accD	4	CGTGAAGCTTGCAAATGGAG	CTCAGCCCACACCTATGTTC	20	20	50.0	55.0	58.0	58.0	2.1	0.0	0.056700	56645	57467	987-987	0.500000	59.5
rbcL-accD_p5	rbcL-accD	5	GGCTTCAGGGGGTATTCATG	GCCCACACCTATGTTCTAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.058191	56645	57467	1188-1188	0.500000	59.5
accD_p1	accD	1	ATCCGATGGATCCTTGGGAT	ATGAGAATTGGTTCCGTCGG	20	20	50.0	50.0	57.9	58.0	28.4	4.4	0.074930	57468	58931	2398-2398	0.500000	59.4
accD_p2	accD	2	ATCCGATGGATCCTTGGGAT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	28.4	26.9	0.077757	57468	58931	2335-2335	0.500000	59.4
accD_p3	accD	3	ATCCGATGGATCCTTGGGAT	TGTGTTCGAAAAGAGCCACA	20	20	50.0	45.0	57.9	58.0	28.4	11.6	0.082013	57468	58931	2489-2489	0.500000	59.3
accD_p4	accD	4	ATCCGATGGATCCTTGGGAT	GGGCACAAAAATGGAGGGTA	20	20	50.0	50.0	57.9	58.1	28.4	0.0	0.119119	57468	58931	2301-2301	0.500000	59.0
accD_p5	accD	5	GGGCTCCTTCTACTCATCCT	ATGAGAATTGGTTCCGTCGG	20	20	55.0	50.0	57.9	58.0	0.0	4.4	0.119780	57468	58931	2326-2326	0.500000	59.0
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AGTCAGTTAACAGCCAACCG	AGGAAGAGATAAGAGGGCCC	20	20	50.0	55.0	58.1	57.9	8.6	16.5	0.231800	109499	110074	709-726	0.500000	60.0
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	AGTCAGTTAACAGCCAACCG	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	58.1	57.9	8.6	29.2	0.231800	109499	110074	708-725	0.500000	60.0
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	ACGACCAGTCAGTTAACAGC	AGGAAGAGATAAGAGGGCCC	20	20	50.0	55.0	57.8	57.9	8.6	16.5	0.261150	109499	110074	715-732	0.500000	59.8
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	ACGACCAGTCAGTTAACAGC	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	57.8	57.9	8.6	29.2	0.261150	109499	110074	714-731	0.500000	59.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	AGGATTCGAACCTACGACCA	GGAAGAGATAAGAGGGCCCT	20	20	50.0	55.0	58.2	57.9	15.0	29.2	0.267385	109499	110074	727-744	0.500000	59.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCTGTGGGAATAATCAGA	AGAAAGAGCTTTTACTGCAGT	22	21	40.9	38.1	57.7	55.4	0.0	25.1	5.858880	112520	113290	938-970	1.000000	45.3
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCTGTGGGAATAATCAGA	AAGAAAGAGCTTTTACTGCAGT	22	22	40.9	36.4	57.7	56.2	0.0	25.1	6.094687	112520	113290	939-971	1.000000	45.3
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGCTGTGGGAATAATCAGA	AGAAAGAGCTTTTACTGCAGTT	22	22	40.9	36.4	57.7	56.2	0.0	27.1	6.094687	112520	113290	938-970	1.000000	45.3
ndhF-rpl32_p4	ndhF-rpl32	4	TGCTGTGGGAATAATCAGAAGT	AGAAAGAGCTTTTACTGCAGT	22	21	40.9	38.1	57.4	55.4	0.0	25.1	6.133968	112520	113290	935-967	1.000000	45.3
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCTGTGGGAATAATCAGA	GCTAAAGAAAGAGCTTTTACTGCA	22	24	40.9	37.5	57.7	58.0	0.0	0.0	6.301648	112520	113290	943-975	1.000000	45.3
ycf1_p1	ycf1	1	ATTTTGCGTCTTTGAGCACG	ACGGAAAAACTATGCCACGA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.209408	127976	128095	1106-1118	1.000000	90.3
ycf1_p2	ycf1	2	ATTTTGCGTCTTTGAGCACG	TGCCTGCCCAAAATGTATGA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.265101	127976	128095	929-941	1.000000	89.9
ycf1_p3	ycf1	3	AAAAGTGGGGAATGCACACT	ACGGAAAAACTATGCCACGA	20	20	45.0	45.0	57.9	57.8	3.1	0.0	0.259462	127976	128095	1151-1151	0.500000	57.9
ycf1_p4	ycf1	4	TCTCCTGTTTCGCCTTTTCC	ACGGAAAAACTATGCCACGA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.280642	127976	128095	787-787	0.500000	57.8
ycf1_p5	ycf1	5	CTCAGCCTGCATTTTCTCCT	ACGGAAAAACTATGCCACGA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.298624	127976	128095	810-810	0.500000	57.6
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	CCTTCTTCCATAACCCGAGC	GAGCGAGCCCTTTAATTCCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.083460	129836	130411	822-994	1.000000	90.1
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	CCTTCTTCCATAACCCGAGC	TAGCATAGCAAAGACGCGTT	20	20	55.0	45.0	58.0	58.0	0.0	15.5	0.049656	129836	130411	1086-1258	1.000000	88.4
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	CCTTCTTCCATAACCCGAGC	GTAACCCAGGCCAGAATCAG	20	20	55.0	55.0	58.0	58.0	0.0	5.0	0.089196	129836	130411	1182-1359	1.000000	87.4
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	CCTTCTTCCATAACCCGAGC	TCGAACTTGGGTAGGGTCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.064880	129836	130411	1268-1445	1.000000	87.2
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	CCTTCTTCCATAACCCGAGC	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.080603	129836	130411	1117-1134	0.500000	60.6
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CCAAATTCGGGGGTCAAAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.091329	152864	153023	300-406	1.000000	91.3
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	CTCCAAATTCGGGGGTCAAA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.091329	152864	153023	302-408	1.000000	91.3
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	TCCAAATTCGGGGGTCAAAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.1	58.0	0.0	7.6	0.091329	152864	153023	301-407	1.000000	91.3
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.036329	152864	153023	889-929	0.500000	59.7
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CGAACCCCGGTTAGATTCAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.1	58.0	0.0	7.6	0.073268	152864	153023	726-766	0.500000	59.4