Hotspot

petD

LSC · 72978-74195 · 1218 bp

Back to Trigonotis

Pi 0.00679

MarkerSeek score 47.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1218
variable_sites: 29
pi: 0.00679
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTCAACTCGCCTC	TCCCTAGATACACACGTCGT	50.0	50.0	57.9	57.6	1330-1353 bp	1.0	79.8
petD_p2	TTGATATGAAGCGTTGGGGG	TCCCTAGATACACACGTCGT	50.0	50.0	57.9	57.6	1305-1328 bp	0.928571	77.5
petD_p3	CCCTTCAACTCGCCTCTTTA	TCCCTAGATACACACGTCGT	50.0	50.0	57.2	57.6	1326-1349 bp	1.0	74.6
petD_p4	TCCCTTCAACTCGCCTCTTT	TCCCTAGATACACACGTCGT	50.0	50.0	58.9	57.6	1327-1350 bp	1.0	73.2
petD_p5	TTGATATGAAGCGTTGGGGG	TTCCCTAGATACACACGTCGT	50.0	47.6	57.9	58.3	1306-1329 bp	0.928571	70.5