Hotspot

ndhA

SSC · 114333-116520 · 2188 bp

Back to Trigonotis

Pi 0.00173

MarkerSeek score 52.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2188
variable_sites: 23
pi: 0.00173
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTCAGTTACCATAGGGAACA	CCGCCGGGTTTTATCAATTT	42.9	45.0	55.7	57.0	2384-2435 bp	1.0	48.8
ndhA_p2	ACTCAGTTACCATAGGGAACA	GCCGGGTTTTATCAATTTGCA	42.9	42.9	55.7	58.0	2382-2433 bp	1.0	48.1
ndhA_p3	GAACTCAGTTACCATAGGGAACA	CCGCCGGGTTTTATCAATTT	43.5	45.0	57.6	57.0	2386-2437 bp	1.0	47.9
ndhA_p4	TGAACTCAGTTACCATAGGGAAC	CCGCCGGGTTTTATCAATTT	43.5	45.0	57.6	57.0	2387-2438 bp	1.0	47.9
ndhA_p5	GAACTCAGTTACCATAGGGAACA	GCCGGGTTTTATCAATTTGCA	43.5	42.9	57.6	58.0	2384-2435 bp	1.0	47.2