Hotspot

ndhF-rpl32

SSC · 107627-108292 · 666 bp

Back to Trigonotis

Pi 0.00646

MarkerSeek score 56.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 666
variable_sites: 24
pi: 0.00646
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAAGGGTATGATCCACGCAT	CCCCCTTTTCCAAATATTTTTACGA	50.0	36.0	57.7	58.1	616-805 bp	1.0	46.6
ndhF-rpl32_p2	AAGCAAGGGTATGATCCACG	CCCCCTTTTCCAAATATTTTTACGA	50.0	36.0	57.7	58.1	619-808 bp	1.0	46.6
ndhF-rpl32_p3	TCGGAAAAAGAAGAAGCCCC	CCCCCTTTTCCAAATATTTTTACGA	50.0	36.0	58.1	58.1	668-857 bp	1.0	46.5
ndhF-rpl32_p4	TCGGAAAAAGAAGAAGCCCC	CCCCCTTTTCCAAATATTTTTACG	50.0	37.5	58.1	56.8	668-857 bp	1.0	46.5
ndhF-rpl32_p5	AAAAGAAGAAGCCCCACTCC	CCCCCTTTTCCAAATATTTTTACGA	50.0	36.0	57.7	58.1	663-852 bp	1.0	46.5