Hotspot

trnS-UGA-psbZ

LSC · 34670-35047 · 378 bp

Back to Trigonotis

Pi 0.00799

MarkerSeek score 42.70

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 14
pi: 0.00799
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	AATTGGAAAGCAAGAGTCAT	55.0	35.0	58.0	53.0	402-440 bp	1.0	46.7
trnS-UGA-psbZ_p2	TCAACCACTCAGCCATCTCT	AATTGGAAAGCAAGAGTCAT	50.0	35.0	58.4	53.0	403-441 bp	1.0	46.7
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	AATTGGAAAGCAAGAGTCAT	50.0	35.0	57.9	53.0	422-460 bp	1.0	46.6
trnS-UGA-psbZ_p4	GCTATCAACCACTCAGCCAT	AATTGGAAAGCAAGAGTCAT	50.0	35.0	57.7	53.0	407-445 bp	1.0	46.6
trnS-UGA-psbZ_p5	TTTTCAAGACCGGGGCTATC	AATTGGAAAGCAAGAGTCAT	50.0	35.0	57.6	53.0	421-459 bp	1.0	46.6