Hotspot

rpoB-trnC-GCA

LSC · 26498-27629 · 1132 bp

Back to Trigonotis

Pi 0.00657

MarkerSeek score 47.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 34
pi: 0.00657
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	AGGCAATAATTCGGCGAGTT	50.0	45.0	58.0	58.0	1772-1807 bp	1.0	85.2
rpoB-trnC-GCA_p2	TGTTCTTGCATATCCCTGCC	AGGCAATAATTCGGCGAGTT	50.0	45.0	57.9	58.0	1550-1585 bp	1.0	85.2
rpoB-trnC-GCA_p3	CCGGGGCTTTGCAATATTTG	AGGCAATAATTCGGCGAGTT	50.0	45.0	58.1	58.0	1652-1687 bp	1.0	85.1
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1697-1728 bp	1.0	84.9
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	CTATACAAACCCCACCCAGC	50.0	55.0	58.0	57.9	1842-1885 bp	0.892857	80.7