Hotspot

atpH-atpI

LSC · 13227-14316 · 1090 bp

Back to Trigonotis

Pi 0.00189

MarkerSeek score 55.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 17
pi: 0.00189
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	1170-1234 bp	1.0	83.1
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TAGCTGCGGCGTATATAGGT	45.0	50.0	58.1	58.2	1159-1223 bp	1.0	82.9
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1188-1252 bp	1.0	82.7
atpH-atpI_p4	TACCCTCTACAGCTTGACCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.1	57.9	1255-1319 bp	1.0	82.5
atpH-atpI_p5	GCGATACCCTCTACAGCTTG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.9	57.9	1259-1323 bp	1.0	82.5