Hotspot

trnS-GCU-trnG-UCC

LSC · 8120-8711 · 592 bp

Back to Trigonotis

Pi 0.00584

MarkerSeek score 56.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 18
pi: 0.00584
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGACCCT	45.0	42.9	58.0	57.5	661-957 bp	1.0	73.3
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGACCCT	50.0	42.9	58.2	57.5	710-1006 bp	1.0	72.4
trnS-GCU-trnG-UCC_p3	ACAACAACGGAAAGAGAGGG	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.7	57.5	716-1012 bp	1.0	71.5
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.6	57.5	647-943 bp	1.0	70.4
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGACCCT	55.0	42.9	58.5	57.5	642-938 bp	1.0	69.6