Hotspot

rps19

LSC · 85050-85328 · 279 bp

Back to Triglochin

Pi 0.00717

MarkerSeek score 43.30

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 2
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TCGGGCTTTGAAAACAGACA	CGTTTTATTCTTCGTCGCCG	45.0	50.0	58.0	57.9	721-870 bp	1.0	83.7
rps19_p2	TCGGGCTTTGAAAACAGACA	GGGTAGAGCCGGATCTAAGT	45.0	55.0	58.0	58.0	908-1057 bp	1.0	83.3
rps19_p3	TCGGGCTTTGAAAACAGACA	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.0	58.0	909-1058 bp	1.0	83.3
rps19_p4	TCGGGCTTTGAAAACAGACA	GAGCCGGATCTAAGTGTTGG	45.0	55.0	58.0	58.1	903-1052 bp	1.0	82.8
rps19_p5	TCGGGCTTTGAAAACAGACA	GAGGGTAAATCGGCCACATT	45.0	50.0	58.0	57.9	1021-1170 bp	1.0	82.5