Hotspot

rpl16

LSC · 82114-83587 · 1474 bp

Back to Triglochin

Pi 0.0183

MarkerSeek score 57.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1474
variable_sites: 27
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCCAATATCTATATATCCATATCC	ACCATTTTATTTGGTGAAATTGCT	32.0	29.2	52.9	56.3	1523-1531 bp	1.0	41.4
rpl16_p2	ACCCAATATCTATATATCCATATCC	ACCATTTTATTTGGTGAAATTGC	32.0	30.4	52.9	54.9	1523-1531 bp	1.0	41.4
rpl16_p3	ACCCAATATCTATATATCCATATCC	CCATTTTATTTGGTGAAATTGCT	32.0	30.4	52.9	54.6	1522-1530 bp	1.0	41.4
rpl16_p4	ACCCAATATCTATATATCCATATCC	TCGATTGACCATTTTATTTGG	32.0	33.3	52.9	52.5	1530-1538 bp	1.0	41.4
rpl16_p5	ACCCAATATCTATATATCCATATCC	TTCGATTGACCATTTTATTTGG	32.0	31.8	52.9	53.4	1531-1539 bp	1.0	41.4