Hotspot

trnR-ACG-trnN-GUU

IRb · 109466-110068 · 603 bp

Back to Triglochin

Pi 0.00542

MarkerSeek score 65.80

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 603
variable_sites: 3
pi: 0.00542
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	695-744 bp	1.0	82.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	646-695 bp	1.0	81.4
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CGGTCGGCTGTTAACTGATT	55.0	50.0	57.8	58.3	665-714 bp	1.0	80.4
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	689-738 bp	1.0	80.1
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GGTTCGAATCCTACTTGGGG	55.0	55.0	57.8	57.7	638-687 bp	1.0	80.0