Hotspot

rps19-rpl2

LSC · 85329-85454 · 126 bp

Back to Triglochin

Pi 0.037

MarkerSeek score 64.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 4
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTTAGCCACAAAAGGATT	CGTTTTATTCTTCGTCGCCG	40.0	50.0	55.1	57.9	194-324 bp	1.0	61.8
rps19-rpl2_p2	TGGTTAGCCACAAAAGGATT	CCCCTTGGGGTTATCCTACA	40.0	55.0	55.1	58.1	253-383 bp	1.0	61.2
rps19-rpl2_p3	TGGTTAGCCACAAAAGGATTT	CGTTTTATTCTTCGTCGCCG	38.1	50.0	55.9	57.9	194-324 bp	1.0	60.4
rps19-rpl2_p4	TGGTTAGCCACAAAAGGATTT	CCCCTTGGGGTTATCCTACA	38.1	55.0	55.9	58.1	253-383 bp	1.0	59.8
rps19-rpl2_p5	TGGTTAGCCACAAAAGGATT	AAAAACCCATGACCCCTTGG	40.0	50.0	55.1	58.3	265-395 bp	1.0	59.7