Hotspot

rpl22-rps19

LSC · 84845-85049 · 205 bp

Back to Triglochin

Pi 0.07

MarkerSeek score 73.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 14
pi: 0.07
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCGATCTCTTCCAGGTCTTC	TGGTAGACATGCGAGAAACG	55.0	50.0	57.8	58.0	273-292 bp	1.0	83.6
rpl22-rps19_p2	TAGGCGATCTCTTCCAGGTC	TGGTAGACATGCGAGAAACG	55.0	50.0	58.3	58.0	276-295 bp	1.0	82.8
rpl22-rps19_p3	ATAGGCGATCTCTTCCAGGT	TGGTAGACATGCGAGAAACG	50.0	50.0	57.6	58.0	277-296 bp	1.0	82.1
rpl22-rps19_p4	GCGATCTCTTCCAGGTCTTC	GGAGAATTCGCACCTACTCG	55.0	55.0	57.8	58.2	299-318 bp	1.0	81.9
rpl22-rps19_p5	GCGATCTCTTCCAGGTCTTC	GGGAGAATTCGCACCTACTC	55.0	55.0	57.8	57.8	300-319 bp	1.0	81.4