Hotspot

trnM-CAU-atpE

LSC · 53409-53554 · 146 bp

Back to Triglochin

Pi 0.0345
MarkerSeek score 68.30
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
146
variable_sites
5
pi
0.0345
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 CACAAAGCAGTCGGTCTCTT GGTCGAGGCTATCAATGCAA 50.0 50.0 58.1 58.0 292-294 bp 1.0 80.2
trnM-CAU-atpE_p2 TTCACAAAGCAGTCGGTCTC GGTCGAGGCTATCAATGCAA 50.0 50.0 58.1 58.0 294-296 bp 1.0 80.2
trnM-CAU-atpE_p3 TATTGCTTTCATACGGCGGG GGTCGAGGCTATCAATGCAA 50.0 50.0 58.4 58.0 226-228 bp 1.0 78.5
trnM-CAU-atpE_p4 ATTCACAAAGCAGTCGGTCT GGTCGAGGCTATCAATGCAA 45.0 50.0 57.4 58.0 295-297 bp 1.0 76.8
trnM-CAU-atpE_p5 ACAAAGCAGTCGGTCTCTTT GGTCGAGGCTATCAATGCAA 45.0 50.0 57.4 58.0 291-293 bp 1.0 76.3