Hotspot

rps4-trnT-ACA

LSC · 46962-47353 · 392 bp

Back to Triglochin

Pi 0.0204

MarkerSeek score 64.20

Primer available yes

Label rps4-trnT-ACA

Feature Metadata

label_name: rps4-trnT-ACA
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 8
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-ACA_p1	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	59.1	57.9	522-544 bp	1.0	71.4
rps4-trnT-ACA_p2	AGTTAGTCCCGGTAAAGCCC	GATGGTCATCGGTTCGACTC	55.0	55.0	59.1	58.2	491-513 bp	1.0	71.3
rps4-trnT-ACA_p3	TCACTTCCGGATCTAGGTCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	56.9	57.9	548-570 bp	1.0	71.1
rps4-trnT-ACA_p4	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	52.6	55.0	58.1	57.9	504-526 bp	1.0	71.1
rps4-trnT-ACA_p5	TCACTTCCGGATCTAGGTCT	GATGGTCATCGGTTCGACTC	50.0	55.0	56.9	58.2	517-539 bp	1.0	70.9