Hotspot

psbI-trnS-GCU

LSC · 8482-8593 · 112 bp

Back to Triglochin

Pi 0.0561

MarkerSeek score 73.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 6
pi: 0.0561
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGATGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.7	58.0	203-208 bp	1.0	81.6
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGATGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.7	58.0	204-209 bp	1.0	81.6
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGATGAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.7	58.2	153-158 bp	1.0	81.3
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGATGAA	TTCGAATCCCTCTCTCTCCG	50.0	55.0	57.7	58.3	150-155 bp	1.0	80.6
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGATGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.7	58.1	222-227 bp	1.0	80.4