Hotspot

rps16-trnQ-UUG

LSC · 6025-7092 · 1068 bp

Back to Triglochin

Pi 0.0282

MarkerSeek score 67.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1068
variable_sites: 29
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TGGTCCTGTTATTCGGAGGT	45.0	50.0	58.1	58.1	1156-1157 bp	1.0	83.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1141-1142 bp	1.0	83.0
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1145-1146 bp	1.0	78.2
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACCGGTTTTGGTC	45.0	50.0	58.1	58.7	1171-1172 bp	1.0	78.0
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	TGGTCCTGTTATTCGGAGGT	50.0	50.0	57.2	58.1	1148-1149 bp	1.0	77.9