Hotspot

trnH-GUG-psbA

LSC · 65-318 · 254 bp

Back to Triglochin

Pi 0.0315

MarkerSeek score 66.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 8
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTAGCTGGTGTTGAAGCTCC	55.0	50.0	58.1	57.8	311-323 bp	1.0	79.5
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CTGGTGTTGAAGCTCCTTCT	55.0	50.0	58.1	57.4	307-319 bp	1.0	76.6
trnH-GUG-psbA_p3	CGCATGGTGGATTCACAATC	TTAGCTGGTGTTGAAGCTCC	50.0	50.0	57.5	57.8	340-352 bp	1.0	76.5
trnH-GUG-psbA_p4	GATTCACAATCCACCGCCTT	TTAGCTGGTGTTGAAGCTCC	50.0	50.0	58.5	57.8	331-343 bp	1.0	76.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TAGCTGGTGTTGAAGCTCCT	55.0	50.0	58.1	58.6	310-322 bp	1.0	75.8