Hotspot

tRNA-Asn-tRNA-Arg

Genome · 104757-105201 · 445 bp

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Pi 0.302

MarkerSeek score 77.70

Primer available yes

Label tRNA-Asn-tRNA-Arg

Feature Metadata

label_name: tRNA-Asn-tRNA-Arg
feature_type: igs
region: Genome
length_bp: 445
variable_sites: 175
pi: 0.302
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn-tRNA-Arg_p1	CCGTTGTTCCTCAGTAGCTC	ATAGATAGGGGTGGAAGGGC	55.0	55.0	58.0	58.0	1096 bp	0.333333	52.9
tRNA-Asn-tRNA-Arg_p2	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGCATCCTAACAG	55.0	55.0	58.0	58.0	1241 bp	0.333333	52.9
tRNA-Asn-tRNA-Arg_p3	CCGTTGTTCCTCAGTAGCTC	GCTGAGGCATCCTAACAGAC	55.0	55.0	58.0	58.1	1239 bp	0.333333	52.9
tRNA-Asn-tRNA-Arg_p4	ATTTGCCGTTGTTCCTCAGT	ATAGATAGGGGTGGAAGGGC	45.0	55.0	58.0	58.0	1101 bp	0.333333	52.8
tRNA-Asn-tRNA-Arg_p5	CCGTTGTTCCTCAGTAGCTC	GGTGACGATACTGTAGGGGA	55.0	55.0	58.0	57.9	911 bp	0.333333	52.8