Hotspot

tRNA-Gln-tRNA-Trp

Genome · 81313-91440 · 10128 bp

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Pi 0.32

MarkerSeek score 74.30

Primer available yes

Label tRNA-Gln-tRNA-Trp

Feature Metadata

label_name: tRNA-Gln-tRNA-Trp
feature_type: igs
region: Genome
length_bp: 10128
variable_sites: 4181
pi: 0.32
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gln-tRNA-Trp_p1	GGGACCCCCTCAAATTCATC	GAGCTCGACATGTCTGGAAG	55.0	55.0	57.9	58.1	880 bp	0.333333	52.1
tRNA-Gln-tRNA-Trp_p2	GAACAGCCAACCATCGTACT	GAGCTCGACATGTCTGGAAG	50.0	55.0	57.9	58.1	322 bp	0.333333	51.9
tRNA-Gln-tRNA-Trp_p3	GGGACCCCCTCAAATTCATC	GAAGCACAGGAGAACGTTCT	55.0	50.0	57.9	57.8	864 bp	0.333333	51.5
tRNA-Gln-tRNA-Trp_p4	GAACAGCCAACCATCGTACT	GAAGCACAGGAGAACGTTCT	50.0	50.0	57.9	57.8	306 bp	0.333333	51.3
tRNA-Gln-tRNA-Trp_p5	GGGACCCCCTCAAATTCATC	AACCAAGAAAGAGCCGTAGC	55.0	50.0	57.9	58.2	1503 bp	0.333333	51.2