Hotspot

tRNA-Gly-tRNA-Gly

Genome · 78253-78935 · 683 bp

Back to Trifolium

Pi 0.3

MarkerSeek score 79.40

Primer available yes

Label tRNA-Gly-tRNA-Gly

Feature Metadata

label_name: tRNA-Gly-tRNA-Gly
feature_type: igs
region: Genome
length_bp: 683
variable_sites: 255
pi: 0.3
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gly-tRNA-Gly_p1	CATCGTTAGCTTGGAAGGCT	CAGCCATCTCTCCCCAATTC	50.0	55.0	58.0	58.0	1440 bp	0.333333	52.9
tRNA-Gly-tRNA-Gly_p2	CATCGTTAGCTTGGAAGGCT	ATGAGATTCGCCCCCTTTTC	50.0	50.0	58.0	57.9	878 bp	0.333333	52.6
tRNA-Gly-tRNA-Gly_p3	CATCGTTAGCTTGGAAGGCT	TAGGTACTGGCATGGCTCTT	50.0	50.0	58.0	58.1	1164 bp	0.333333	52.1
tRNA-Gly-tRNA-Gly_p4	CATCGTTAGCTTGGAAGGCT	GAGATTCGCCCCCTTTTCTT	50.0	50.0	58.0	57.9	876 bp	0.333333	52.1
tRNA-Gly-tRNA-Gly_p5	CATCGTTAGCTTGGAAGGCT	ACCAGAGTCCAGCTAGGTAC	50.0	55.0	58.0	57.9	1177 bp	0.333333	51.9