Hotspot

tRNA-Thr-tRNA-Glu

Genome · 55697-56469 · 773 bp

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Pi 0.322

MarkerSeek score 81.50

Primer available yes

Label tRNA-Thr-tRNA-Glu

Feature Metadata

label_name: tRNA-Thr-tRNA-Glu
feature_type: igs
region: Genome
length_bp: 773
variable_sites: 333
pi: 0.322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr-tRNA-Glu_p1	GAACCGATGACTTACGCCTT	ATCCCGATCGGAATTTTGCA	50.0	45.0	58.0	57.9	1195 bp	0.333333	52.9
tRNA-Thr-tRNA-Glu_p2	GAACCGATGACTTACGCCTT	TTTGTATCCGAGCCCATTGG	50.0	50.0	58.0	57.9	1117 bp	0.333333	52.9
tRNA-Thr-tRNA-Glu_p3	TACCGCTGAGTTAAAAGGGC	ATCCCGATCGGAATTTTGCA	50.0	45.0	57.9	57.9	1160 bp	0.333333	52.1
tRNA-Thr-tRNA-Glu_p4	TACCGCTGAGTTAAAAGGGC	TTTGTATCCGAGCCCATTGG	50.0	50.0	57.9	57.9	1082 bp	0.333333	52.1
tRNA-Thr-tRNA-Glu_p5	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	915 bp	0.333333	52.0