Hotspot

tRNA-Ser-tRNA-Thr

Genome · 51560-55624 · 4065 bp

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Pi 0.34

MarkerSeek score 76.00

Primer available yes

Label tRNA-Ser-tRNA-Thr

Feature Metadata

label_name: tRNA-Ser-tRNA-Thr
feature_type: igs
region: Genome
length_bp: 4065
variable_sites: 1795
pi: 0.34
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Thr_p1	CGAGCACCTCCTAGCATTTT	TTGCTACTCCTCTCCTCCAC	50.0	55.0	58.0	58.1	884 bp	0.333333	51.9
tRNA-Ser-tRNA-Thr_p2	CGAGCACCTCCTAGCATTTT	CTCCGCAAGAATCTGTCGAT	50.0	50.0	58.0	57.8	945 bp	0.333333	51.4
tRNA-Ser-tRNA-Thr_p3	GATGGAACCTTCGAGGCTTT	TTGCTACTCCTCTCCTCCAC	50.0	55.0	57.9	58.1	860 bp	0.333333	51.2
tRNA-Ser-tRNA-Thr_p4	CGAGCACCTCCTAGCATTTT	CACGCGGAAGGACTTATTCT	50.0	50.0	58.0	57.7	867 bp	0.333333	50.7
tRNA-Ser-tRNA-Thr_p5	GATGGAACCTTCGAGGCTTT	CTCCGCAAGAATCTGTCGAT	50.0	50.0	57.9	57.8	921 bp	0.333333	50.7