Hotspot

tRNA-Leu-tRNA-Thr

Genome · 43502-44764 · 1263 bp

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Pi 0.304
MarkerSeek score 82.80
Primer available yes
Label tRNA-Leu-tRNA-Thr

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu-tRNA-Thr_p1 ACCAATTTCGCCATATCCCC CAGTTAGTCCGGGTAAAGCC 50.0 55.0 58.0 58.0 1692-1739 bp 1.0 91.8
tRNA-Leu-tRNA-Thr_p2 ACCAATTTCGCCATATCCCC CCAGTTAGTCCGGGTAAAGC 50.0 55.0 58.0 58.0 1693-1740 bp 1.0 91.8
tRNA-Leu-tRNA-Thr_p3 ACCAATTTCGCCATATCCCC CGAAGCCATACCCAATCGAA 50.0 50.0 58.0 58.1 1922-1978 bp 1.0 91.5
tRNA-Leu-tRNA-Thr_p4 ACCAATTTCGCCATATCCCC TTTTCCAGCGATACGAACGT 50.0 45.0 58.0 57.9 1838-1894 bp 1.0 91.4
tRNA-Leu-tRNA-Thr_p5 ACCAATTTCGCCATATCCCC TTGACCAGTTGACCCTTTGG 50.0 50.0 58.0 57.9 1915 bp 0.333333 52.8