Hotspot

rps15-ycf1

SSC · 124455-125751 · 1297 bp

Back to Tricyrtis

Pi 0.017

MarkerSeek score 63.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 1297
variable_sites: 22
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAAATGCGAGGTGAGTCTCC	AGTCAATATAAAAACATCAACGT	55.0	26.1	58.6	52.8	1490-1492 bp	1.0	41.3
rps15-ycf1_p2	CAAATGCGAGGTGAGTCTCC	AAACCTCCTATATTATCGAATCA	55.0	30.4	58.6	52.6	1454-1455 bp	1.0	41.3
rps15-ycf1_p3	CAAATGCGAGGTGAGTCTCC	AACCTCCTATATTATCGAATCAA	55.0	30.4	58.6	52.6	1453-1454 bp	1.0	41.3
rps15-ycf1_p4	CAAATGCGAGGTGAGTCTCC	ACCTCCTATATTATCGAATCAAA	55.0	30.4	58.6	52.6	1452-1453 bp	1.0	41.3
rps15-ycf1_p5	CAAATGCGAGGTGAGTCTCC	ACCTCCTATATTATCGAATCAAAAAGA	55.0	29.6	58.6	56.5	1452-1453 bp	1.0	41.3