Hotspot

rps3-rpl22

LSC · 84446-84510 · 65 bp

Back to Tricyrtis

Pi 0.0357

MarkerSeek score 70.30

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTTGTGCGAACCAAAAGGAA	AGTTAAGACCTCGGGCTAGA	45.0	50.0	57.4	57.2	256-260 bp	1.0	72.3
rps3-rpl22_p2	TGTGCGAACCAAAAGGAATG	AGTTAAGACCTCGGGCTAGA	45.0	50.0	57.2	57.2	254-258 bp	1.0	70.5
rps3-rpl22_p3	GTTGTGCGAACCAAAAGGAA	AAGTTAAGACCTCGGGCTAGA	45.0	47.6	57.4	57.9	257-261 bp	1.0	70.1
rps3-rpl22_p4	TCTTCCTGTATACCCACGGA	AGTTAAGACCTCGGGCTAGA	50.0	50.0	57.2	57.2	287-291 bp	1.0	69.8
rps3-rpl22_p5	GTTGTGCGAACCAAAAGGAA	GTTAAGACCTCGGGCTAGAG	45.0	55.0	57.4	56.8	255-259 bp	1.0	69.6