Hotspot

petD-rpoA

LSC · 78636-78926 · 291 bp

Back to Tricyrtis

Pi 0.0597

MarkerSeek score 72.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 16
pi: 0.0597
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CATGCAGTAAGTATAAGTATCTAG	ACCCTACAGAAGCATTTCGC	33.3	50.0	52.2	58.3	348-369 bp	1.0	47.6
petD-rpoA_p2	CATGCAGTAAGTATAAGTATCTAG	CCCTACAGAAGCATTTCGCA	33.3	50.0	52.2	58.3	347-368 bp	1.0	47.6
petD-rpoA_p3	CATGCAGTAAGTATAAGTATCTAG	ACAGAAGCATTTCGCAATCG	33.3	45.0	52.2	57.5	343-364 bp	1.0	47.6
petD-rpoA_p4	CATGCAGTAAGTATAAGTATCTAG	CAGAAGCATTTCGCAATCGA	33.3	45.0	52.2	57.2	342-363 bp	1.0	47.6
petD-rpoA_p5	CATGCAGTAAGTATAAGTATCTAG	ACACCCTACAGAAGCATTTCG	33.3	47.6	52.2	58.3	350-371 bp	1.0	47.6