Hotspot

petA-psbJ

LSC · 63371-64221 · 851 bp

Back to Tricyrtis

Pi 0.0261

MarkerSeek score 69.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 22
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1003-1007 bp	1.0	79.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	999-1003 bp	1.0	78.6
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1002-1006 bp	1.0	78.6
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1000-1004 bp	1.0	78.4
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	996-1000 bp	1.0	77.5