Hotspot

accD-psaI

LSC · 58951-59832 · 882 bp

Back to Tricyrtis

Pi 0.0125

MarkerSeek score 53.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 11
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGCTCCACGGTTTCTTTC	AAGGCACAAAAATAGAGGGT	50.0	40.0	58.1	54.8	959-987 bp	1.0	54.5
accD-psaI_p2	TCAGCTCCACGGTTTCTTTC	AGGCACAAAAATAGAGGGTAGA	50.0	40.9	58.1	56.8	958-986 bp	1.0	54.4
accD-psaI_p3	TCAGCTCCACGGTTTCTTTC	AAAGGCACAAAAATAGAGGGT	50.0	38.1	58.1	55.6	960-988 bp	1.0	53.2
accD-psaI_p4	TTCAGCTCCACGGTTTCTTT	AAGGCACAAAAATAGAGGGT	45.0	40.0	57.7	54.8	960-988 bp	1.0	52.7
accD-psaI_p5	TTTCAGCTCCACGGTTTCTT	AAGGCACAAAAATAGAGGGT	45.0	40.0	57.7	54.8	961-989 bp	1.0	52.7