Hotspot

trnT-GGU-psbD

LSC · 32320-33338 · 1019 bp

Back to Tricyrtis

Pi 0.00901
MarkerSeek score 51.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1019
variable_sites
9
pi
0.00901
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGAACTGGCTAATCCATGGG 55.0 55.0 58.1 58.0 1275-1278 bp 1.0 81.3
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GAACAGATCGAGCAAGCTCA 55.0 50.0 58.1 58.0 1486-1489 bp 1.0 81.2
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TGAACAGATCGAGCAAGCTC 55.0 50.0 58.1 58.0 1487-1490 bp 1.0 81.2
trnT-GGU-psbD_p4 ACCCCCTCCATCCCTAAAAA GAACAGATCGAGCAAGCTCA 50.0 50.0 57.9 58.0 2047-2051 bp 1.0 80.8
trnT-GGU-psbD_p5 ACCCCCTCCATCCCTAAAAA TGAACAGATCGAGCAAGCTC 50.0 50.0 57.9 58.0 2048-2052 bp 1.0 80.8