Hotspot

atpH-atpI

LSC · 14381-15274 · 894 bp

Back to Tricyrtis

Pi 0.00796

MarkerSeek score 64.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 894
variable_sites: 7
pi: 0.00796
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	980-982 bp	1.0	77.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	953-955 bp	1.0	76.8
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1036-1038 bp	1.0	76.7
atpH-atpI_p4	AACGGAAGCGGCAGAAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	978-980 bp	1.0	76.2
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.9	57.4	1009-1011 bp	1.0	76.1