Hotspot

trnR-UCU-atpA

LSC · 10637-10737 · 101 bp

Back to Tricyrtis

Pi 0.0396

MarkerSeek score 62.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 4
pi: 0.0396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAAAGGCGTCCATTGTCT	GTTTCTACTTCAGGAACAAACA	45.0	36.4	57.5	54.5	205-217 bp	1.0	42.3
trnR-UCU-atpA_p2	ATGAAAGGCGTCCATTGTCT	GAACTGTTTCTACTTCAGGAAC	45.0	40.9	57.5	54.7	210-222 bp	1.0	42.2
trnR-UCU-atpA_p3	AGGAATGAAAGGCGTCCATT	GAACTGTTTCTACTTCAGGAAC	45.0	40.9	57.5	54.7	214 bp	0.5	20.0
trnR-UCU-atpA_p4	GGAATGAAAGGCGTCCATTG	GAACTGTTTCTACTTCAGGAAC	50.0	40.9	57.4	54.7	213 bp	0.5	20.0
trnR-UCU-atpA_p5	AGGAATGAAAGGCGTCCATT	GTTTCTACTTCAGGAACAAACA	45.0	36.4	57.5	54.5	209 bp	0.5	20.0