Hotspot

trnS-GCU-trnG-UCC

LSC · 8480-9673 · 1194 bp

Back to Tricyrtis

Pi 0.0207

MarkerSeek score 65.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1194
variable_sites: 24
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1248-1268 bp	1.0	55.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1251-1271 bp	1.0	54.2
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1250-1270 bp	1.0	54.2
trnS-GCU-trnG-UCC_p4	GAGGGATAGCGGAGAGAGAG	ACGAATCACACTTTTACCACT	60.0	38.1	58.2	55.5	1307-1327 bp	1.0	53.9
trnS-GCU-trnG-UCC_p5	ATGAGGGATAGCGGAGAGAG	ACGAATCACACTTTTACCACT	55.0	38.1	57.8	55.5	1309-1329 bp	1.0	53.6