Hotspot

rps16-trnQ-UUG

LSC · 5906-7382 · 1477 bp

Back to Tricyrtis

Pi 0.0103
MarkerSeek score 65.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1477
variable_sites
15
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1548-1551 bp 1.0 80.0
rps16-trnQ-UUG_p2 TCCACAGTCGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1570-1573 bp 1.0 79.9
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1559-1562 bp 1.0 79.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1552-1555 bp 1.0 78.9
rps16-trnQ-UUG_p5 TCCACAGTCGATCATGTCCT CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.1 57.8 1574-1577 bp 1.0 78.9