Hotspot

rps15-ycf1

SSC · 125751-126148 · 398 bp

Back to Trichosanthes

Pi 0.0303

MarkerSeek score 71.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 398
variable_sites: 39
pi: 0.0303
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTTGCGAAATAACTGAGCTGA	AGCAGTCGTTTTAGTATGCT	40.9	40.0	58.1	54.8	491-500 bp	1.0	50.5
rps15-ycf1_p2	TCTTGCGAAATAACTGAGCTGA	GCAGTCGTTTTAGTATGCTAAGG	40.9	43.5	58.1	57.6	490-499 bp	1.0	50.5
rps15-ycf1_p3	CTTGCGAAATAACTGAGCTGA	AGCAGTCGTTTTAGTATGCT	42.9	40.0	56.6	54.8	490-499 bp	1.0	50.5
rps15-ycf1_p4	TCTTGCGAAATAACTGAGCTG	AGCAGTCGTTTTAGTATGCT	42.9	40.0	56.6	54.8	491-500 bp	1.0	50.5
rps15-ycf1_p5	CTTGCGAAATAACTGAGCTGA	GCAGTCGTTTTAGTATGCTAAGG	42.9	43.5	56.6	57.6	489-498 bp	1.0	50.5