Hotspot

rpl32-trnL

SSC · 115304-116379 · 1076 bp

Back to Trichosanthes

Pi 0.0327

MarkerSeek score 76.10

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 1076
variable_sites: 138
pi: 0.0327
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	1076-1428 bp	1.0	88.5
rpl32-trnL_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	1077-1429 bp	1.0	88.5
rpl32-trnL_p3	GGCAGCGTTGAAAGCTTTTT	GGAGAGCGGGGACTAATAGT	45.0	55.0	58.4	58.0	1629-1983 bp	1.0	87.7
rpl32-trnL_p4	GGCAGCGTTGAAAGCTTTTT	TGGAGAGCGGGGACTAATAG	45.0	55.0	58.4	58.0	1630-1984 bp	1.0	87.7
rpl32-trnL_p5	GGCAGCGTTGAAAGCTTTTT	CGGGGACTAATAGTGGGGAT	45.0	55.0	58.4	58.0	1623-1977 bp	1.0	87.6