Hotspot

ndhF-rpl32

SSC · 114475-115141 · 667 bp

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Pi 0.0263

MarkerSeek score 75.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 667
variable_sites: 61
pi: 0.0263
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCCGTCGGAAAAAGTAGAA	CGCCCTTTGCTTTTCCAAAT	45.0	45.0	57.5	57.8	815-837 bp	1.0	84.5
ndhF-rpl32_p2	TTGCCGTCGGAAAAAGTAGA	CGCCCTTTGCTTTTCCAAAT	45.0	45.0	57.5	57.8	816-838 bp	1.0	84.5
ndhF-rpl32_p3	GAAGGTTTTTGGTTGCCGTC	CGCCCTTTGCTTTTCCAAAT	50.0	45.0	58.2	57.8	828-850 bp	0.928571	84.1
ndhF-rpl32_p4	GTCGAAGGTTTTTGGTTGCC	CGCCCTTTGCTTTTCCAAAT	50.0	45.0	58.2	57.8	831-853 bp	0.928571	84.1
ndhF-rpl32_p5	CGTCGAAGGTTTTTGGTTGC	CGCCCTTTGCTTTTCCAAAT	50.0	45.0	58.5	57.8	832-854 bp	0.928571	81.1