Hotspot

trnT-trnL

LSC · 48663-49638 · 976 bp

Back to Trichosanthes

Pi 0.0189

MarkerSeek score 64.50

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 59
pi: 0.0189
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	911-1060 bp	1.0	85.8
trnT-trnL_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	943-1092 bp	1.0	84.8
trnT-trnL_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	905-1054 bp	1.0	84.6
trnT-trnL_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	943-1092 bp	1.0	83.6
trnT-trnL_p5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	937-1086 bp	1.0	83.5