Hotspot

ycf3-trnS

LSC · 46646-47261 · 616 bp

Back to Trichosanthes

Pi 0.0136

MarkerSeek score 65.60

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 28
pi: 0.0136
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	CCTCTTTTTCCCCGGAAGTT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.7	58.1	568-791 bp	1.0	83.9
ycf3-trnS_p2	CCTCTTTTTCCCCGGAAGTT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.7	58.2	589-812 bp	1.0	83.3
ycf3-trnS_p3	TGAATGCCTCTTTTTCCCCG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.5	58.1	574-797 bp	1.0	82.5
ycf3-trnS_p4	TGAATGCCTCTTTTTCCCCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.5	58.2	595-818 bp	1.0	82.0
ycf3-trnS_p5	CCTCTTTTTCCCCGGAAGTT	ACCCTCGGTAAACAAAAGCC	50.0	50.0	57.7	58.4	569-792 bp	1.0	81.5