Hotspot

psaA-ycf3

LSC · 43901-44643 · 743 bp

Back to Trichosanthes

Pi 0.0123

MarkerSeek score 63.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 41
pi: 0.0123
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	ATCAAGCCGCTGAGTATTGG	61.1	50.0	58.4	58.0	850-869 bp	1.0	67.3
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	AGCCGCTGAGTATTGGAAAC	61.1	50.0	58.4	58.3	846-865 bp	1.0	65.5
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	CAAGCCGCTGAGTATTGGAA	61.1	50.0	58.4	58.3	848-867 bp	1.0	65.5
psaA-ycf3_p4	ACTTCTGGTTCCGGCGAA	ATCAAGCCGCTGAGTATTGG	55.6	50.0	58.9	58.0	851-870 bp	1.0	63.9
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	CGGAGGCTTGGTTCAATCAA	61.1	50.0	58.4	58.5	865-884 bp	1.0	63.7