Hotspot

trnE-trnT

LSC · 32574-33507 · 934 bp

Back to Trichosanthes

Pi 0.0188

MarkerSeek score 72.40

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 58
pi: 0.0188
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1025-1133 bp	1.0	86.9
trnE-trnT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	1004-1112 bp	1.0	86.5
trnE-trnT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	1001-1109 bp	1.0	86.3
trnE-trnT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	987-1095 bp	1.0	86.2
trnE-trnT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	966-1074 bp	1.0	86.2