Hotspot

atpF-atpH

LSC · 14152-14479 · 328 bp

Back to Trichosanthes

Pi 0.00774

MarkerSeek score 64.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 12
pi: 0.00774
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAGTAACCCAAAGA	GGACTGGTTGTAGCATTAGCA	50.0	47.6	57.5	58.0	411-453 bp	1.0	73.8
atpF-atpH_p2	GATGGCCAGTAACCCAAAGA	TGGACTGGTTGTAGCATTAGC	50.0	47.6	57.5	58.0	412-454 bp	1.0	73.8
atpF-atpH_p3	GGATGGCCAGTAACCCAAAG	GGACTGGTTGTAGCATTAGCA	55.0	47.6	58.5	58.0	412-454 bp	1.0	73.7
atpF-atpH_p4	GGATGGCCAGTAACCCAAAG	TGGACTGGTTGTAGCATTAGC	55.0	47.6	58.5	58.0	413-455 bp	1.0	73.6
atpF-atpH_p5	GATGGCCAGTAACCCAAAGA	GGAGGGAAAAATCCGAGGTAC	50.0	52.4	57.5	58.1	475-517 bp	1.0	72.6